Isolation of Arcobacter butzleri and A. cryaerophilus in Samples of Meats and from Meat-Processing Plants by a Culture Technique and Detection by PCR

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F03%3A00000614" target="_blank" >RIV/00216275:25310/03:00000614 - isvavai.cz</a>

Alternative codes found

RIV/00216275:25310/03:00006599

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Isolation of Arcobacter butzleri and A. cryaerophilus in Samples of Meats and from Meat-Processing Plants by a Culture Technique and Detection by PCR

Original language description

A pilot survey of sources of contamination with arcobacters, which represent a potential risk for humans, is described. A wide range of samples involved various kinds of meat (beef, pork, meat products, chilled chickens, etc.), both from a retail level and domestic farming. Sanitary practices in abattoirs and production lines were checked in two different plants (a beef and pork production and a chicken processing plant). The applied method was based on a selective enrichment to isolate suspect strains,in combination with a PCR technique specific for arcobacters. The choice of a suitable enrichment broth and a plating agar was made with the use of pure bacterial strains and by means of real meat samples seeded with Arcobacter butzleri. The PCR technique was optimized to allow differentiation of a 1223 bp product, typical for the genus Arcobacter, and a product of 686 bp, specific for A. butzleri. On the whole, a total number of 198 samples was tested, of that 33 (16.7%) were found to

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GA203%2F02%2F0023" target="_blank" >GA203/02/0023: Development of new procedures for highly reliable analytical control of technologies,materials,food &environment & for med. diagnostic using modern instrumentat</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Volume of the periodical

48

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

5

Pages from-to

227-232

UT code for WoS article

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EID of the result in the Scopus database

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