Comparison of aflatoxinogenic fungal DNA isolation for PCR.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F06%3A00004295" target="_blank" >RIV/00216275:25310/06:00004295 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Comparison of aflatoxinogenic fungal DNA isolation for PCR.
Original language description
Two classical techniques of DNA extraction were optimized and compared with use of commercial PCR kits. For DNA isolation was also used commercially delivered compound DNAzol(R)ES. Methods were optimized by using pure cultures A. parasiticus var. globosus CCM F - 550 and A. flavus CCM F - 108. These methods were classified in view of DNA purity, concentration, speed of isolation, intensity of instrument techniques and manipulation and last but not least were taken single isolation cost into consideration. The most effective method for DNA isolation was chosen for positive samples for the presence of the aflatoxinogenic fungi. 83 samples of food (first of all tea, spices and medical herbs) were examined. 27 samples were positive for the presence of theaflatoxinogenic fungi on the AFPA medium. After DNA isolation was made amplification by PCR and detection by electrophoresis. Acquired result practically corresponded with result gained on the AFPA medium.
Czech name
Porovnání jednotlivých metod izolace DNA z aflatoxinogenních plísní.
Czech description
určeno pro seznam publikací
Classification
Type
D - Article in proceedings
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA203%2F05%2F2106" target="_blank" >GA203/05/2106: Modern instrumentation in chemical, food and clinical analysis</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
20th International ICFMH Symposium Food Micro 2006
ISBN
961-90346-3-5
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
453
Publisher name
International Committee on Food Microbiology and Hygiene
Place of publication
Bologna, Itálie
Event location
Bologna
Event date
Aug 29, 2006
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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