Comparison of fungal DNA isolation method for subsequent PCR

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F07%3A00005920" target="_blank" >RIV/00216275:25310/07:00005920 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Srovnání metod izolace plísňové DNA pro následnou PCR

Original language description

Two classical techniques of DNA extraction were optimized and compared with use of commercial PCR kits. For DNA isolation was also used commercially delivered compound DNAzol?ES. Methods were optimized by using pure cultures A. parasiticus var. globosusCCM F ? 550 and A. flavus CCM F ? 108. These methods were classified in view of DNA purity, concentration, speed of isolation, intensity of instrument techniques and manipulation and last but not least were taken single isolation cost into consideration.The most effective method for DNA isolation was chosen for positive samples for the presence of the aflatoxinogenic fungi. 83 samples of food (first of all tea, spices and medical herbs) were examined. 27 samples were positive for the presence of the aflatoxinogenic fungi on the AFPA medium. After DNA isolation was made amplification by PCR and detection by electrophoresis. Acquired result practically corresponded with result gained on the AFPA medium.

Czech name

Srovnání metod izolace plísňové DNA pro následnou PCR

Czech description

Two classical techniques of DNA extraction were optimized and compared with use of commercial PCR kits. For DNA isolation was also used commercially delivered compound DNAzol?ES. Methods were optimized by using pure cultures A. parasiticus var. globosusCCM F ? 550 and A. flavus CCM F ? 108. These methods were classified in view of DNA purity, concentration, speed of isolation, intensity of instrument techniques and manipulation and last but not least were taken single isolation cost into consideration.The most effective method for DNA isolation was chosen for positive samples for the presence of the aflatoxinogenic fungi. 83 samples of food (first of all tea, spices and medical herbs) were examined. 27 samples were positive for the presence of the aflatoxinogenic fungi on the AFPA medium. After DNA isolation was made amplification by PCR and detection by electrophoresis. Acquired result practically corresponded with result gained on the AFPA medium.

Type

D - Article in proceedings

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA203%2F05%2F2106" target="_blank" >GA203/05/2106: Modern instrumentation in chemical, food and clinical analysis</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Sborník IX. konference mladých vědeckých pracovníků s mezinárodní účastí

ISBN

978-80-7305-012-2

ISSN

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e-ISSN

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Number of pages

4

Pages from-to

55-58

Publisher name

Veterinární a farmaceutická univerzita Brno

Place of publication

Brno

Event location

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Event date

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Type of event by nationality

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UT code for WoS article

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