Detection of aflatoxinogenic fungi in plants

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F08%3A00007609" target="_blank" >RIV/00216275:25310/08:00007609 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Detection of aflatoxinogenic fungi in plants
Original language description
The study examined occurrence of fungi in food. 91 samples of food (first of all tea, spices and medical herbs) were examined. 27 samples were positive for the presence of the aflatoxinogenic fungi on the AFPA medium. Two samples contained two species fungi of genus Aspergillus and one sample contained three species fungi of genus Aspergillus. Finally we determined 31 kinds of the potentially aflatoxinogenic fungi on the AFPA medium. The PCR were positive for 27 kinds of the aflatoxinogenic fungi. Isolated Aspergillus strains were examined using PCR method. Method was optimized by using pure cultures A. parasiticus var. globosus CCM F ? 550 and A. flavus CCM F ? 108. Specific PCR product (apa-2 gene and ver-1 gene) codes the biosynthetic way of aflatoxin B1. PCR product was detected by electrophoresis.
Czech name
DETEKCE POTENCIÁLNĚ AFLATOXINOGENNÍCH PLÍSNÍ V ROSTLINÝCH MATERIÁLECH
Czech description
Práce se zabývá detekcí plísní rodu Aspergillus, které jsou jedním z nejčastějších kontaminantů potravin. Zamoření potravin ale i krmiv jak plísněmi rodu Aspergillus, tak jejich toxickými metabolity je závažný problém, neboť mají nepříznivý účinek na zdraví lidí a živočichů.

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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