Extraction of DNA to detection of aflatoxigenic aspergillus species in foodstuffs using PCR method

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F07%3A00005935" target="_blank" >RIV/00216275:25310/07:00005935 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Extraction of DNA to detection of aflatoxigenic aspergillus species in foodstuffs using PCR method

Original language description

The extraction of DNA according to Cenis was used with different modifications of disrupting the cell wall. Fungi mycelium was disrupted by ultrasonic bath, Eppendorf homogenizer and liquid nitrogen. Method was optimized using pure cultures A. parasiticus var. globosus CCM F ? 550 and A. flavus CCM F ? 108. After extraction, acquired DNA was amplified by Polymerase Chain Reaction (PCR). PCR was used to amplify ver-1 gene and apa-2 gene as target fragments. The specific PCR product codes the biosyntheticway of aflatoxin B1. PCR product was detected by electrophoresis. Records were interpreted. Acquired results demonstrate, that success of method depends especially on disrupting cell wall of fungi. 91 samples of food (first of all tea, spices and medical herbs) were examined. 27 samples were positive for the presence of the aflatoxinogenic fungi on the AFPA medium. Two samples contained two species fungi of genus Aspergillus and one sample contained three species fungi of genus Aspergil

Czech name

Extrakce DNA z aflatoxinogenních druhů aspergilů vyskytujících se v potravinách pro PCR

Czech description

Extrakce DNA z aflatoxinogenních druhů aspergilů vyskytujících se v potravinách pro následnou polymerázovou řetězovou reakci.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scientific Papers of the University of Pardubice, Series A

ISSN

1211-5541

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

13

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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