Isocratic HPLC: System Selection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F09%3A00008429" target="_blank" >RIV/00216275:25310/09:00008429 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Isocratic HPLC: System Selection

Original language description

The effects of various factors on the separation in isocratic HPLC are discussed. Various separation modes, including aqueous-organic and non-aqueous reversedphase (RP) chromatography, adsorption normal-phase liquid chromatography (NPLC), hydrophilic interaction liquid chromatography (HILIC), ion-exchange, ion-pair, and size-exclusion chromatography (SEC) for non-ionic samples differing in the size and polarities and for partially or fully ionized acidic or basic compounds are addressed. Selection of the column length, inner diameter (I.D.), character of the column bed, and the type of stationary phase are discussed, using the information on the sample properties, such as solubility, polarity, presence of specific functional groups in sample compounds,sample amount and concentration, and so on. Empirical and model-based approaches to the optimization of the operation parameters controlling the separation selectivity, efficiency, and resolution in HPLC are outlined.

Czech name

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Czech description

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Type

C - Chapter in a specialist book

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name

Encyclopedia of Chromatography

ISBN

978-1-4200-8459-7

Number of pages of the result

11

Pages from-to

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Number of pages of the book

2850

Publisher name

CRC Press

Place of publication

London

UT code for WoS chapter

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