Comparison of various modes and phase systems for analytical HPLC
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F20%3A39916970" target="_blank" >RIV/00216275:25310/20:39916970 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/B978-0-444-64070-3.00001-1" target="_blank" >http://dx.doi.org/10.1016/B978-0-444-64070-3.00001-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/B978-0-444-64070-3.00001-1" target="_blank" >10.1016/B978-0-444-64070-3.00001-1</a>
Alternative languages
Result language
angličtina
Original language name
Comparison of various modes and phase systems for analytical HPLC
Original language description
This chapter addresses principles of the separation modes used in contemporary column liquid chromatography, including reversed-phase organic normal-phase hydrophilic interaction liquid chromatography (aqueous normal-phase chromatography) and ion-exchange separation systems, with respect to the effects of the stationary and mobile phases and the experimental conditions on the separation. This treatment compares the advantages and disadvantages of various types and sizes of column particles, column formats and dimensions on the efficiency of separation, with special attention to the sub-2 μm particles, core-shell and monolithic columns. Theoretical retention models for the individual separation modes deal with the selection of the mobile phase in the isocratic retention models and possible extension to gradient elution separations. Temperature affects the sample diffusivity and hence the separation efficiency on one side, and the thermodynamic contributions of enthalpy and entropy to the energy of retention on the other. Serial, heart-cutting and comprehensive two-dimensional techniques combining independent (orthogonal) separation modes largely increase the peak capacity of liquid chromatography.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
C - Předmět řešení projektu podléhá obchodnímu tajemství (§ 504 Občanského zákoníku), ale název projektu, cíle projektu a u ukončeného nebo zastaveného projektu zhodnocení výsledku řešení projektu (údaje P03, P04, P15, P19, P29, PN8) dodané do CEP, jsou upraveny tak, aby byly zveřejnitelné.
Data specific for result type
Book/collection name
Handbook of Analytical Separations. Vol. 8
ISBN
978-0-444-64070-3
Number of pages of the result
91
Pages from-to
1-91
Number of pages of the book
788
Publisher name
Elsevier Science BV
Place of publication
Amsterdam
UT code for WoS chapter
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