Inhibition of acetylcholinesterase by 14 achiral and five chiral imidazole derivates

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F10%3A39881510" target="_blank" >RIV/00216275:25310/10:39881510 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Inhibition of acetylcholinesterase by 14 achiral and five chiral imidazole derivates

Original language description

Homological series of 14 nonchiral derivates and series of 5 chiral derivates of imidazole were tested in vitro as inhibitors of hydrolysis of acetylcholine using enzyme preparation of acetylcholine esterase from electric eel. The batch stirred reactor at 25°C, pH 8 (phosphate buffer), ionic strength 0.11 M and catalytic activity of the enzyme preparation 0.14 U ml-1 of the reaction mixture were used. The temporal dependences of actual concentrations of acetylcholine, choline and acetic acid were determined by an original HPLC method. For all used inhibitors, these time dependences conform with the probability of more than 90 % to the model of competitive irreversible inhibition. All kinetic constants including k3 defining the rate of inhibition (0.71- 5.3 M-1 s-1) and qualified estimation of the absolute acetylcholine esterase concentration in the reaction mixture (40 - 110 nM) were determined.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CF - Physical chemistry and theoretical chemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bioresource Technology

ISSN

0960-8524

e-ISSN

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Volume of the periodical

101

Issue of the periodical within the volume

15

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

3

Pages from-to

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UT code for WoS article

000278035900080

EID of the result in the Scopus database

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