Separation of Phenolic Acids and Flavone Natural Antioxidants by Two-Dimensional Method Combining Liquid Chromatography and Micellar Electrokinetic Capillary Chromatography
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F10%3A39882327" target="_blank" >RIV/00216275:25310/10:39882327 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Separation of Phenolic Acids and Flavone Natural Antioxidants by Two-Dimensional Method Combining Liquid Chromatography and Micellar Electrokinetic Capillary Chromatography
Original language description
A 2-D method was developed for separation of phenolic acids and flavone compounds combining LC with MEKC. The effect of substituted neutral and anionic CD additives to the background electrolyte on the quality of MEKC separation was investigated. The best selectivity of the MEKC separation was achieved in 25 mmol/L borate background buffer at pH 9.05 with the addition of 10 g/L SDS and 1.85 g/L heptakis (6-O-sulfo)-b-CD. These conditions were used in the second dimension of 2-D combination of LC and MEKC separation in combination with a PEG column in the first dimension, providing the best orthogonality (the lowest degree of correlation between the selectivity of separation) in the two dimensions. A CE autosampler was employed as the interface betweenLC and MEKC steps based on automated fraction collection before the re-analysis of the collected LC fractions in the second, MEKC dimension.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
31
Issue of the periodical within the volume
13
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
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UT code for WoS article
000280226900013
EID of the result in the Scopus database
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