Improvement of the sensitivity of 2D LC-MEKC separation of phenolic acids and flavonoids natural antioxidants using the on-line preconcentration step
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F12%3A39883550" target="_blank" >RIV/00216275:25310/12:39883550 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/elps.201100594" target="_blank" >http://dx.doi.org/10.1002/elps.201100594</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201100594" target="_blank" >10.1002/elps.201100594</a>
Alternative languages
Result language
angličtina
Original language name
Improvement of the sensitivity of 2D LC-MEKC separation of phenolic acids and flavonoids natural antioxidants using the on-line preconcentration step
Original language description
A 2D method was developed for separation of phenolic acids and flavonoids natural antioxidants combining LC with MEKC. The in-capillary preconcentration step was applied for the improvement of the sensitivity of 2D method before the second dimension MEKCanalysis. The influence of first dimension LC mobile phase composition on migration times in the second MEKC dimension was evaluated. When gradient elution is applied in the first dimension of 2D LC-MEKC system, increasing concentration of organic solvent in the mobile phase and in fractions transferred from LC influences the electroosmotic flow, partitioning equilibria of samples in micelles and properties of the micelles, which results in shifts of migration times during the consecutive runs in the second MEKC separation dimension. The shifts of migration times caused by the influence of increasing concentration of ACN on MEKC separation in second dimension of 2D LC-MEKC system were compensated by aligning the time axis using electro
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
33
Issue of the periodical within the volume
15
Country of publishing house
DE - GERMANY
Number of pages
10
Pages from-to
2464-2473
UT code for WoS article
000307399400026
EID of the result in the Scopus database
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