Lipidomic Profiling of Biological Tissues Using Off-line Two-dimensional High-performance Liquid Chromatography Mass Spectrometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892094" target="_blank" >RIV/00216275:25310/11:39892094 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.chroma.2011.05.081" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2011.05.081</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2011.05.081" target="_blank" >10.1016/j.chroma.2011.05.081</a>

Result language
angličtina
Original language name
Lipidomic Profiling of Biological Tissues Using Off-line Two-dimensional High-performance Liquid Chromatography Mass Spectrometry
Original language description
In this work, we describe a new method for the characterization of the lipid composition in various tissues, using off-line two-dimensional coupling of HILIC and RP-HPLC coupled to ESI and APCI mass spectrometry. In the first dimension the total lipid extracts are fractioned using HILIC into individual lipid classes. In total, 19 lipid classes (+3 regioisomeric pairs) that cover a wide range of polarities are separated in one analytical run, which is the highest number of analyzed lipid classes reportedso far. The lysophospholipid regioisomers are also separated in HILIC mode followed by the identification based on the characteristic ESI mass spectra. The collected fractions of the various lipid classes are further separated in the RP mode, which offers an excellent resolution of the individual lipid species. Their ESI or APCI mass spectra give correct information on the fatty acid composition and on the individual regioisomeric positions on the glycerol skeleton.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
<a href="/en/project/GP203%2F09%2FP249" target="_blank" >GP203/09/P249: New stationary Phases for Separation of Lipids</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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