Lipidomic analysis of porcine organs using hydrophilic interaction liquid chromatography and mass spectroscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39898169" target="_blank" >RIV/00216275:25310/14:39898169 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Lipidomic analysis of porcine organs using hydrophilic interaction liquid chromatography and mass spectroscopy
Original language description
The identification and quantitation of all lipids in complex biological tissues is the first step towards the understanding how lipids function in a biological system and the elucidation of the mechanism of lipid-related diseases including obesity, atherosclerosis, cancer, cardiovascular diseases, etc. Our optimized nontargeted method using hydrophilic interaction liquid chromatography (HILIC) coupled to electrospray ionization mass spectrometry (ESI-MS) was used for the characterization of the lipidomemainly glycerophospholipids and sphingolipids in porcine brain, heart, kidney, liver, lung, spinal cord, spleen and stomach. Individual lipid classes are quantified based on the peak integration of individual lipid classes separated in the HILIC mode multiplied by their response factors and correlated by sphingosylethanolamine (d17:1/12:0) as a single internal standard. Subsequently, relative abundances of deprotonated molecules [M-H]- in negative-ion ESI mass spectra are used for deter
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
ISSN
1211-5541
e-ISSN
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Volume of the periodical
20
Issue of the periodical within the volume
září 2014
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
14
Pages from-to
23-36
UT code for WoS article
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EID of the result in the Scopus database
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