Determination of serum creatinine in selected groups of patients by means of HPLC with UV detection - comparison with Jaffé and enzymatic assays
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892308" target="_blank" >RIV/00216275:25310/11:39892308 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Determination of serum creatinine in selected groups of patients by means of HPLC with UV detection - comparison with Jaffé and enzymatic assays
Original language description
Results from measurements of by a high-performance liquid chromatography on 232 serum samples were compared with enzymatic and Jaffé methods. The serum samples were deproteinized with ethanol. For the separation, a column MAG 1, 250 mm x 4.6 mm, Labiospher PSI 100 C18, 5 mým, was used. The mixture of ethanol and 25 mmol l-1 sodium hydrogenphosphate (3:97 v/v), pH 6.5 was used as a mobile phase. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 6 %. Quantitative recoveries of spiked serum samples were between 101.8 and 106.0 %. The limit of quantification was 10.0 mýmol l-1. The results obtained by chromatographic method correlated well with Jaffé assays. Enzymatic method gaveat average significantly higher values depending on a group of patients.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
ISSN
1211-5541
e-ISSN
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Volume of the periodical
17
Issue of the periodical within the volume
2011
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
14
Pages from-to
57-70
UT code for WoS article
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EID of the result in the Scopus database
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