An assay of creatine in human serum using an HPLC with UV detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896728" target="_blank" >RIV/00216275:25310/13:39896728 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
An assay of creatine in human serum using an HPLC with UV detection
Original language description
An ion-exchange high-performance liquid chromatography with ultraviolet detection for the determination of creatine in human serum has been developed. The human serum was deproteinized with perchloric acid. For the separation, a cation-exchange column LC-SCX, 250 mm x 4.6 mm, 5 um, was used. The mixture of ethanol and 10mmol l-1 ammonium acetate (15:85, v/v), pH 4.00+/-0.05 was used as a mobile phase. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficientsof variation were below 10%. Quantitative recoveries of spiked serum samples were between 97.1% and 103.5%. The calibration curve was linear in the whole range tested. The limit of quantification was 2 umol l-1. The preliminary reference ranges of serumcreatine in a group of blood donors are 17-89 umol l-1 for women and 21-97 umol l-1 for men.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
ISSN
1211-5541
e-ISSN
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Volume of the periodical
19
Issue of the periodical within the volume
prosinec
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
10
Pages from-to
39-48
UT code for WoS article
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EID of the result in the Scopus database
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