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An assay of creatine in human serum using an HPLC with UV detection

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896728" target="_blank" >RIV/00216275:25310/13:39896728 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    An assay of creatine in human serum using an HPLC with UV detection

  • Original language description

    An ion-exchange high-performance liquid chromatography with ultraviolet detection for the determination of creatine in human serum has been developed. The human serum was deproteinized with perchloric acid. For the separation, a cation-exchange column LC-SCX, 250 mm x 4.6 mm, 5 um, was used. The mixture of ethanol and 10mmol l-1 ammonium acetate (15:85, v/v), pH 4.00+/-0.05 was used as a mobile phase. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficientsof variation were below 10%. Quantitative recoveries of spiked serum samples were between 97.1% and 103.5%. The calibration curve was linear in the whole range tested. The limit of quantification was 2 umol l-1. The preliminary reference ranges of serumcreatine in a group of blood donors are 17-89 umol l-1 for women and 21-97 umol l-1 for men.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology

  • ISSN

    1211-5541

  • e-ISSN

  • Volume of the periodical

    19

  • Issue of the periodical within the volume

    prosinec

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    10

  • Pages from-to

    39-48

  • UT code for WoS article

  • EID of the result in the Scopus database