Temperature dependence of retention in reversed-phase and hydrophilic interaction liquid chromatography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892633" target="_blank" >RIV/00216275:25310/11:39892633 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Temperature dependence of retention in reversed-phase and hydrophilic interaction liquid chromatography

Original language description

The retention mechanism in reversed-phase liquid chromatography (RPLC) and in hydrophilic interaction liquid chromatography (HILIC) have been investigated by examining the temperature dependence of retention of the chosen phenolic acids on two columns modified by hydrosilated silica - hydrosilated silica column, Diamond hydride column, cholesterol column and C18 bidentate column. The linear dependences of retention on temperature were observed in the HILIC as well as in the RPLC on all columns tested. Van't Hoff equation was applied to describe the retention mechanism thoroughly, and its slopes and intercepts were used for calculations of thermodynamic constants for the transfer of a solute from the mobile phase to the stationary phase (deltaH0 and deltaS0).

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA203%2F07%2F0641" target="_blank" >GA203/07/0641: New analytical methods in combined high-capacity phase separation systems</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologija

ISSN

1392-0146

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

2

Country of publishing house

LT - LITHUANIA

Number of pages

7

Pages from-to

85-91

UT code for WoS article

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EID of the result in the Scopus database

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