Assessment of glutathione reductase inhibition

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892670" target="_blank" >RIV/00216275:25310/11:39892670 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Assessment of glutathione reductase inhibition
Original language description
Glutathione reductase is a crucial enzyme for maintaining of intracellular glutathione levels. This enzyme catalyzes the NADPH-dependent reduction of glutathione disulfide to the reduced form (GSH). The aim of our study was to estimate a possible inhibitory effect on glutathione reductase activity in the presence of two substances that cause glutathione depletion - ethacrynic acid and diethyl maleate. We also tested glutathione as a possible inhibitor. GR activity was determined using spectrophotometricmethod based on measurement of absorbance decline (lambda = 340 nm) due to oxidation of NADPH. We found dose dependent inhibitory effect through reduced glutathione - the presence of 10 mM GSH caused a decrease in enzyme activity by 60%. We conclude that our finding of inhibitory effect in presence of glutathione is of great importance since the GSH levels are very high in the cells.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical
Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
ISSN
1211-5541
e-ISSN
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Volume of the periodical
17
Issue of the periodical within the volume
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Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
7
Pages from-to
77-83
UT code for WoS article
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EID of the result in the Scopus database
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