Inhibitory effect of N-ethylmaleimide in two types of glutathione reductases
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F12%3A39895459" target="_blank" >RIV/00216275:25310/12:39895459 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Inhibitory effect of N-ethylmaleimide in two types of glutathione reductases
Original language description
Glutathione reductase (GR) is a key enzyme of glutathione metabolism. This enzyme catalyzes the NADPH-dependent reduction of glutathione disulfide to a reduced form. The aim of the described study was to estimate an enzyme inhibition in two types of glutathione reductases (human and yeast) through Nethylmaleimide (NEM). The glutathione reductase activity was determined by the spectrophotometric method based on the measurement of an absorbance decline (8 = 340 nm) due to oxidation of NADPH. Interestingly, it was found that the presence of 100 :M NEM had no effect in the two glutathione reductases. The inhibitory effect was proved in higher concentrations of N-ethylmaleimide; however, neither 2 mM NEM was able to diminish GR activity. The enzyme activitywas reduced in both GRs; the human GR was inhibited by 15 % and 37 % in the presence of 1 mM and 2 mM NEM, respectively; the yeast GR was inhibited at the same concentrations of N-ethylmaleimide by 16 % and 35 %, respectively. We assesse
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
—
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
ISSN
1211-5541
e-ISSN
—
Volume of the periodical
18
Issue of the periodical within the volume
prosinec
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
7
Pages from-to
29-35
UT code for WoS article
—
EID of the result in the Scopus database
—