New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F12%3A39894517" target="_blank" >RIV/00216275:25310/12:39894517 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/12:00376785
Result on the web
<a href="http://dx.doi.org/10.1002/mabi.201100393" target="_blank" >http://dx.doi.org/10.1002/mabi.201100393</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/mabi.201100393" target="_blank" >10.1002/mabi.201100393</a>
Alternative languages
Result language
angličtina
Original language name
New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations.
Original language description
Magnetic macroporous PGMA and PHEMA microspheres containing carboxyl groups are synthesized by multi-step swelling and polymerization followed by precipitation of iron oxide inside the pores. The microspheres are characterized by SEM, IR spectroscopy, AAS, and zeta-potential measurements. Their functional groups enable bioactive ligands of various sizes and chemical structures to couple covalently. The applicability of these monodisperse magnetic microspheres in biospecific catalysis and bioaffinity separation is confirmed by coupling with the enzyme trypsin and huIgG. Trypsin-modified magnetic PGMA-COOH and PHEMA-COOH microspheres are investigated in terms of their enzyme activity, operational and storage stability. The presence of IgG molecules on microspheres is confirmed.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Macromolecular Bioscience
ISSN
1616-5187
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
5
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
647-655
UT code for WoS article
000303661300010
EID of the result in the Scopus database
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