RAFT polymerization of N,N-dimethylacrylamide from magnetic poly(2-hydroxyethyl methacrylate) microspheres to suppress nonspecific protein adsorption
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F16%3A00457808" target="_blank" >RIV/61389013:_____/16:00457808 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/pola.27939" target="_blank" >http://dx.doi.org/10.1002/pola.27939</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/pola.27939" target="_blank" >10.1002/pola.27939</a>
Alternative languages
Result language
angličtina
Original language name
RAFT polymerization of N,N-dimethylacrylamide from magnetic poly(2-hydroxyethyl methacrylate) microspheres to suppress nonspecific protein adsorption
Original language description
Nonspecific interaction is a key parameter affecting the efficiency of proteins, nucleic acids or cell separation. Currently, many approaches to introduce antifouling properties to materials have been developed. Among these, surface modification with polymer brushes plays a prominent role. The aim of this study was to synthesize new magnetic microspheres grafted with poly(N,N-dimethylacrylamide) (PDMA) that resist nonspecific protein adsorption. Monodisperse macroporous poly(2-hydroxyethyl methacrylate) (PHEMA) microspheres, 4 µm in size, were synthesized by a multiple swelling polymerization method. To render the microspheres magnetic, iron oxide was precipitated inside the microsphere pores. Functional carboxyl groups, introduced by the hydrolysis of the 2-(methacryloyl)oxyethyl acetate (HEMA-Ac) comonomer, were used to react with propargylamine, followed by coupling of a chain transfer agent via an azide-alkyne click reaction. PDMA was grafted from the PHEMA microspheres using reversible additionfragmentation chain transfer polymerization (RAFT), resulting in surfaces with more than 81 wt % PDMA attached. The successful modification of the microspheres was confirmed by XPS. The magnetic microspheres grafted with PDMA showed excellent antifouling properties as tested in bovine serum protein solutions.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CD - Macromolecular chemistry
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/LH14318" target="_blank" >LH14318: Synthesis of polymer-coated magnetic nanoparticles for biomedicine</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Polymer Science. Part A - Polymer Chemistry
ISSN
0887-624X
e-ISSN
—
Volume of the periodical
54
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
1036-1043
UT code for WoS article
000371890200002
EID of the result in the Scopus database
2-s2.0-84960436071