An assay of 4-hydroxy-trans-2-nonenal in human seminal plasma using a high performance liquid chromatography with fluorescence detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F12%3A39894982" target="_blank" >RIV/00216275:25310/12:39894982 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
An assay of 4-hydroxy-trans-2-nonenal in human seminal plasma using a high performance liquid chromatography with fluorescence detection
Original language description
A method is described for the determination of 4-hydroxy-trans-2-nonenal in human seminal plasma. Semen samples were obtained from male partners of couples presenting for a fertility evaluation. After liquefaction, the samples were centrifuged and the seminal plasma was stored at -80 °C. 4-Hydroxy-trans-2-nonenal was derivatized with 1,3-cyclohexanedione to generate the fluorescent alkyl acridine derivative. After derivatization, seminal plasma proteins were precipitated with cold perchloric acid, and the supernatant was injected into the HPLC system. The separation was realized on an analytical reversed-phase column with fluorescence detection. The mixture of ethanol and deionized water was used as the mobile phase. The intra-assay coefficients of variation were below 10 %. Quantitative recoveries from spiked seminal plasma were between 62.0 and 81.0 %.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Papers of the University of Pardubice, Series A, Faculty of Chemical Technology
ISSN
1211-5541
e-ISSN
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Volume of the periodical
18
Issue of the periodical within the volume
prosinec
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
12
Pages from-to
17-28
UT code for WoS article
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EID of the result in the Scopus database
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