Application of Electrochemical Sensors for Determination of Anticholinesterase Activity and Immobilization of Enzyme

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F12%3A39895541" target="_blank" >RIV/00216275:25310/12:39895541 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Application of Electrochemical Sensors for Determination of Anticholinesterase Activity and Immobilization of Enzyme
Original language description
Cholinesterases are important enzymes, which are responsible for the rapid elimination of acetyclholine after its releasing at cholinergic synapses. High activity of cholinesterases is present in patients with Alzheimer's disease. Therefore, testing of new inhibitors of cholinesterases are important. For determination of the inhibition efficiency several methods can be used. In our case, the anticholinesterase activity expressed by IC50 of five carbamate compounds were tested using screen-printed electrodes and square-wave voltammetry. The next part of our work was focused on testing of two types of immobilization of enzyme on the surface of screen-printed electrodes. Kinetic parameters (Km, Vm) were determined for immobilized and soluted enzyme. Obtained results were disscused.
Czech name
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Czech description
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Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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