The Metabolic Fate of Ivermectin in Host (Ovis aries) and Parasite (Haemonchus contortus)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39895024" target="_blank" >RIV/00216275:25310/13:39895024 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/13:10144279
Result on the web
<a href="http://dx.doi.org/10.1017/S0031182012001680" target="_blank" >http://dx.doi.org/10.1017/S0031182012001680</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1017/S0031182012001680" target="_blank" >10.1017/S0031182012001680</a>

Result language
angličtina
Original language name
The Metabolic Fate of Ivermectin in Host (Ovis aries) and Parasite (Haemonchus contortus)
Original language description
Ivermectin (IVE), one of the most important anthelmintics, is often used in the treatment of haemonchosis in ruminants. The objective of our work was (1) to find and identify phase I and II metabolites of IVE formed by the Barber's pole worm (Haemonchuscontortus), and (2) to compare IVE metabolites in helminths with IVE biotransformation in sheep (Ovis aries) as host species. Ultrahigh-performance liquid chromatography/tandem mass spectrometry (UHPLC/MS/MS) was used for this purpose. During in vitro incubations, microsomes (from adult worms or from ovine liver) and a primary culture of ovine hepatocytes were incubated with IVE. In the ex vivo study, living H. contortus adults were incubated in the presence of 1 mu M IVE for 24 h. The results showed that the H. contortus enzymatic system is not able to metabolize IVE. On the other hand, 7 different phase I as well as 9 phase II IVE metabolites were detected in ovine samples using UHPLC/MS/MS analyses. Most of these metabolites have not
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
<a href="/en/project/GAP502%2F10%2F0217" target="_blank" >GAP502/10/0217: Drug-resistance development in helminths - possible mechanisms and defense</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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