The Inability of Tapeworm Hymenolepis Diminuta and Fluke Dicrocoelium Dendriticum to Metabolize Praziquantel

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F12%3A39892089" target="_blank" >RIV/00216275:25310/12:39892089 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11160/12:10124820

Result on the web

<a href="http://dx.doi.org/10.1016/j.vetpar.2011.09.026" target="_blank" >http://dx.doi.org/10.1016/j.vetpar.2011.09.026</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vetpar.2011.09.026" target="_blank" >10.1016/j.vetpar.2011.09.026</a>

Result language

angličtina

Original language name

The Inability of Tapeworm Hymenolepis Diminuta and Fluke Dicrocoelium Dendriticum to Metabolize Praziquantel

Original language description

Biotransformation enzymes can, to a certain extent, protect parasitic worms against the toxic effects of anthelmintics and can contribute to drug-resistance development. The objective of our work was (1) to find and identify phase I and II metabolites ofthe anthelmintic praziquantel (PZQ) formed by the lancet fluke (Dicrocoelium dendriticum) and the rat tapeworm (Hymenolepis diminuta) and (2) to compare PZQ metabolites in helminths with PZQ biotransformation in rat as host species. Ultra high performance liquid chromatography/tandem mass spectrometry (UHPLC/MS/MS) was used for this purpose. During in vitro incubations, mitochondria-like and microsomes-like fractions (prepared from homogenates of adult worms or from rat liver homogenate) were incubatedwith 10 and 100 mu M PZQ. Liquid/liquid extraction was used for samples during in vitro experiments. In the ex vivo study, living D. dendriticum and H. diminuta adults were incubated in RPMI-1640 medium in the presence of 50 nM or 100 nM

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GAP502%2F10%2F0217" target="_blank" >GAP502/10/0217: Drug-resistance development in helminths - possible mechanisms and defense</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinary Parasitology

ISSN

0304-4017

e-ISSN

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Volume of the periodical

185

Issue of the periodical within the volume

2-4

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

7

Pages from-to

168-174

UT code for WoS article

000302839700017

EID of the result in the Scopus database

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