Gradient Elution in Aqueous Normal-Phase Liquid Chromatography on Hydrosilated Silica-Based Stationary Phases

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896310" target="_blank" >RIV/00216275:25310/13:39896310 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S002196731300366X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S002196731300366X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2013.02.059" target="_blank" >10.1016/j.chroma.2013.02.059</a>

Result language
angličtina
Original language name
Gradient Elution in Aqueous Normal-Phase Liquid Chromatography on Hydrosilated Silica-Based Stationary Phases
Original language description
The possibility of applying a theoretical model in the prediction of the retention of phenolic acids on hydrosilated silica, in aqueous normal phase mode was studied. The actual gradient of the aqueous component in acetonitrile may fluctuate from the pre-set program, as even the gradient-grade acetonitrile contains some water. Hence, the actual concentration of water during the gradient run is higher than pre-set by the gradient program, which leads to lower than expected sample retention. Furthermore,the actual gradient profile may be affected by an increase in water uptake on a polar column during the gradient run. These effects were investigated using the using frontal analysis method and Karl-Fischer titration, for the determination of water in the initial mobile phase, and in the column effluent. Preferential adsorption of water on the Silica hydride, Diamond hydride, UDC Cholesterol, Bidentate C-18, and Phenyl hydride columns can be described by Langmuir isotherms. At the column
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
<a href="/en/project/GAP206%2F12%2F0398" target="_blank" >GAP206/12/0398: Two-dimensional separations in liquid media</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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