Comparison of Nonaqueous Hydrophilic Interaction Chromatography with Aqueous Normal-Phase Chromatography on Hydrosilated Silica-Based Stationary Phases

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896390" target="_blank" >RIV/00216275:25310/13:39896390 - isvavai.cz</a>

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300566/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300566/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201300566" target="_blank" >10.1002/jssc.201300566</a>

Result language

angličtina

Original language name

Comparison of Nonaqueous Hydrophilic Interaction Chromatography with Aqueous Normal-Phase Chromatography on Hydrosilated Silica-Based Stationary Phases

Original language description

We investigated the retention behavior of phenolic acids in nonaqueous normal-phase (NP) LC with buffered methanol/acetonitrile mobile phases on hydrosilated silica-based stationary phases. The silica hydride, Diamond hydride, Bidentate C-18, and Cholesterol columns showed a higher retention of phenolic acids in the nonaqueous mobile phases than in aqueous NP mobile phases. There are some selectivity differences between the aqueous and nonaqueous mobile phases, but generally the resolution and selectivity are better in the aqueous systems. The retention of the phenolic acids tested decreased with increasing concentration of methanol in the mobile phase, up to 20% v/v methanol. At increased temperatures, the retention factors and peak widths decrease inboth NP modes, showing linear ln k versus 1/T plots, due to a single retention mechanism over the temperature range from 25 degrees C up to the column stability limit, however, the best separations are achieved at low temperatures. The e

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GAP206%2F12%2F0398" target="_blank" >GAP206/12/0398: Two-dimensional separations in liquid media</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

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Volume of the periodical

36

Issue of the periodical within the volume

17

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

2753-2759

UT code for WoS article

000323891300006

EID of the result in the Scopus database

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