Possibilities of Retention Prediction in Fast Gradient Liquid Chromatography. Part 1: Comparison of Separation on Packed Fully Porous, Nonporous and Monolithic Columns

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896312" target="_blank" >RIV/00216275:25310/13:39896312 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0021967313000228" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0021967313000228</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2012.12.058" target="_blank" >10.1016/j.chroma.2012.12.058</a>

Result language
angličtina
Original language name
Possibilities of Retention Prediction in Fast Gradient Liquid Chromatography. Part 1: Comparison of Separation on Packed Fully Porous, Nonporous and Monolithic Columns
Original language description
Due to ever increasing importance of short analysis times in modern HPLC practice, fast gradient elution is becoming widely used both in one-dimensional and two-dimensional separations, especially in the second dimension where the speed of separation isof primary importance. For method development and optimization, prediction of retention in gradient elution from the isocratic data is very important. This is principally possible using the well-established theory of gradient elution, especially for reversed-phase separations. Feasibility of these approaches has been proven in conventional liquid chromatography and commercial prediction software is available for this purpose. However, fast gradient chromatography employing short columns and gradient times in between 1 and 2 min or even less, impose additional stringent demands on the accuracy of prediction, due to rapid changes in mobile phase composition and increased role of extra-column contributions and instrumental dwell volume on
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
<a href="/en/project/GAP206%2F12%2F0398" target="_blank" >GAP206/12/0398: Two-dimensional separations in liquid media</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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