Gradient HPLC: Gradient System Selection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F09%3A00008428" target="_blank" >RIV/00216275:25310/09:00008428 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Gradient HPLC: Gradient System Selection

Original language description

Gradient elution is widely used for the separation of complex samples in reversed-phase high-performance liquid chromatography systems and ion-exchange systems. The theory of gradient elution has now been elaborated so that it allows predicting the retention and the resolution of sample compounds in reversed-phase, ion-exchange, and normal-phase systems for almost any combination of gradient profile and relationship between the retention and mobile-phase composition. The most important sources of errorsin the prediction and optimization of gradient elution are the gradient dwell volume and the preferential adsorption of the strong solvents, especially in normal-phase (adsorption) chromatography. The gradient dwell volume effects are more important inmicro-LC techniques than in conventional LC and may cause significant increase in the time of analysis, unless special instrumentation and (or) pre-column flow splitting is used, but their effects can be taken into account in predictive c

Czech name

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Czech description

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Type

C - Chapter in a specialist book

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name

Encyclopedia of Chromatography

ISBN

978-1-4200-8459-7

Number of pages of the result

13

Pages from-to

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Number of pages of the book

2850

Publisher name

CRC Press

Place of publication

London

UT code for WoS chapter

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