Retention behavior of isomeric Triacylglycerols in Silver-ion HPLC: Effects of Mobile Phase Composition and Temperature

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896393" target="_blank" >RIV/00216275:25310/13:39896393 - isvavai.cz</a>

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300550/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300550/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201300550" target="_blank" >10.1002/jssc.201300550</a>

Result language

angličtina

Original language name

Retention behavior of isomeric Triacylglycerols in Silver-ion HPLC: Effects of Mobile Phase Composition and Temperature

Original language description

A systematic study of the retention behavior of isomeric triacylglycerols (TGs) in silver-ion HPLC on a ChromSpher Lipids column has been performed between 10 to 40 degrees C using the most widespread hexane- and dichloromethane-based mobile phases. Therandomization of mono-acyl TG standards and the random esterification of glycerol with fatty acids are employed to produce mixtures of TG isomers. The mobile phase composition has no influence on the general retention pattern, but significant differencesin the retention order of double bond (DB) positional isomers in hexane and dichloromethane mobile phases are described and compared with the previous literature data. Saturated TGs with fatty acyl chain length from C7:0 to C22:0 are partially separatedusing the hexane mobile phase but not at all with the dichloromethane mobile phase. The hexane mobile phase enables at least partial resolution of TG regioisomers with up to seven DBs, while the resolution of only ALA/AAL and ALnA/AALn i

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

—

Project

<a href="/en/project/GA203%2F09%2F0139" target="_blank" >GA203/09/0139: Complex lipidomic characterization of plant and animal tissues</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

—

Volume of the periodical

36

Issue of the periodical within the volume

17

Country of publishing house

DE - GERMANY

Number of pages

13

Pages from-to

2888-2900

UT code for WoS article

000323891300020

EID of the result in the Scopus database

—