The effect of ATM kinase inhibition on the initial response of human dental pulp and periodontal ligament mesenchymal stem cells to ionizing radiation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896447" target="_blank" >RIV/00216275:25310/13:39896447 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/13:10174041 RIV/60162694:G44__/13:43874919
Result on the web
<a href="http://informahealthcare.com/doi/abs/10.3109/09553002.2013.775530" target="_blank" >http://informahealthcare.com/doi/abs/10.3109/09553002.2013.775530</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3109/09553002.2013.775530" target="_blank" >10.3109/09553002.2013.775530</a>

Result language
angličtina
Original language name
The effect of ATM kinase inhibition on the initial response of human dental pulp and periodontal ligament mesenchymal stem cells to ionizing radiation
Original language description
Purpose : This study evaluates early changes in human mesenchymal stem cells (MSC) isolated from dental pulp and periodontal ligament after gamma-irradiation and the effect of ataxia-telangiectasia mutated (ATM) inhibition. Methods : MSC were irradiatedwith 2 and 20 Gy by Co-60. For ATM inhibition, specific inhibitor KU55933 was used. DNA damage was measured by Comet assay and gamma H2AX detection. Cell cycle distribution and proteins responding to DNA damage were analyzed 2-72 h after the irradiation.Results : The irradiation of MSC causes an increase in gamma H2AX; the phosphorylation was ATM-dependent. Irradiation activates ATM kinase, and the level of p53 protein is increased due to its phosphorylation on serine15. While this phosphorylation of p53 is ATM-dependent in MSC, the increase in p53 was not prevented by ATM inhibition. A similar trend was observed for Chk1 and Chk2. The increase in p21 is greater without ATM inhibition. ATM inhibition also does not fully abrogate the ac
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
<a href="/en/project/GA304%2F09%2F1568" target="_blank" >GA304/09/1568: Chatacteristic of resistance of adult stem cells to genotoxic stress</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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