Valproic acid decreases the reparation capacity of irradiated MOLT-4 cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F12%3A10105092" target="_blank" >RIV/00216208:11150/12:10105092 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/12:00389863 RIV/60162694:G44__/12:43874694

Result on the web

<a href="http://www.springerlink.com/content/3101658251154034/fulltext.pdf" target="_blank" >http://www.springerlink.com/content/3101658251154034/fulltext.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1134/S0026893312010141" target="_blank" >10.1134/S0026893312010141</a>

Result language

angličtina

Original language name

Valproic acid decreases the reparation capacity of irradiated MOLT-4 cells

Original language description

The aim of our work was to evaluate mechanisms leading to radiosensitization of MOLT-4 leukemia cells following valproic acid (VA) treatment. Cells were pretreated with 2 mM VA for 24 h followed by irradiation with a dose of 0.5 or 1 Gy. The effect of both noxae, alone and combined, was detected 1 and 24 hours after the irradiation. Induction of apoptosis was evaluated by a flow cytometry. The extent of DNA damage was further determined by phosphorylation of histone H2AX using confocal microscopy. Changes in protein expression were identified by SDS-PAGE/immunoblotting. Two millimolar VA increased apoptosis induction after irradiation as well as phosphorylation of H2AX and provokes an increase in the level of p53 and its phosphorylation at Ser392 in 4h postirradiation. Likewise, p21 protein reached its maximal expression in 4 h after the irradiation of VA treated cells. Twenty four hours later, only the p53 phosphorylated at Ser15 was detected. At the same time, the protein mdm2 (nega

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Biology

ISSN

0026-8933

e-ISSN

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Volume of the periodical

46

Issue of the periodical within the volume

1

Country of publishing house

RU - RUSSIAN FEDERATION

Number of pages

7

Pages from-to

110-116

UT code for WoS article

000302960100013

EID of the result in the Scopus database

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