Dot-ELISA Affinity Test: An Easy, Low-Cost Method to Estimate Binding Activity of Monoclonal Antibodies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F13%3A39896743" target="_blank" >RIV/00216275:25310/13:39896743 - isvavai.cz</a>

Alternative codes found

RIV/61389013:_____/13:00425580

Result on the web

<a href="http://dx.doi.org/10.4172/2155-9872.1000168" target="_blank" >http://dx.doi.org/10.4172/2155-9872.1000168</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4172/2155-9872.1000168" target="_blank" >10.4172/2155-9872.1000168</a>

Result language

angličtina

Original language name

Dot-ELISA Affinity Test: An Easy, Low-Cost Method to Estimate Binding Activity of Monoclonal Antibodies

Original language description

Selecting the right monoclonal antibody (mAb) for an immunoaffinity-based application can be tricky, as many mAb producers offer a wide range of mAb clones against molecular structures of interest. Since there are significant differences in the quality of mAb clones, and particularly in their binding activity, an easy method for quick and low-cost comparison of various mAb clones was developed. The dot-ELISA affinity test is a simple, versatile and instrumentally no demanding technique, since it requires no expensive equipment (such as an ELISA reader or chemiluminescence/fluorescence imaging system) and can be performed in any biochemical laboratory. This method is based on a previously described dot-ELISA technique that is improved with a chaotropicstep using different concentrations of ammonium thiocyanate in the range 0-2 M. In this work, the dot-ELISA affinity test was optimized on Abeta peptide as antigen and anti-Abeta mAb. Such protocol was then applied to a panel of eight ant

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EC - Immunology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Analytical and Bioanalytical Techniques

ISSN

2155-9872

e-ISSN

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Volume of the periodical

4

Issue of the periodical within the volume

3

Country of publishing house

RO - ROMANIA

Number of pages

5

Pages from-to

1-5

UT code for WoS article

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EID of the result in the Scopus database

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