Affiblot: a dot blot-based screening device for selection of reliable antibodies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081715%3A_____%2F21%3A00545112" target="_blank" >RIV/68081715:_____/21:00545112 - isvavai.cz</a>

Alternative codes found

RIV/00216275:25310/21:39918249 RIV/00216208:11160/21:10431693

Result on the web

<a href="http://hdl.handle.net/11104/0321871" target="_blank" >http://hdl.handle.net/11104/0321871</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d1ay00955a" target="_blank" >10.1039/d1ay00955a</a>

Result language

angličtina

Original language name

Affiblot: a dot blot-based screening device for selection of reliable antibodies

Original language description

The key factor in the development of antibody-based assays is to find an antibody that has an appropriate affinity, high specificity, and low cross-reactivity. However, this task is not easy to carry out since the research antibodies on the market may suffer from low specificity and reproducibility. Here, we report on a palm-sized dot blot-based device, called the affiblot, that has a specially designed lid that allows simultaneous semi-quantitative comparison of up to five antibodies from different suppliers regarding their affinity/avidity, cross-reactivity, and batch-to-batch reliability. The only required peripheral equipment is a vacuum pump, a camera, and densitometry software. The affiblot device was tested for its functionality and its measurements were compared against those obtained by standard dot blot and ELISA. The benefit over these methods, when various antibodies are evaluated, is in its simplicity. It allows easy antigen deposition, fast application and the discarding of the solutions, a compact undivided membrane, and therefore significant decrease of labor. The device was tested with specific anti-ApoE, anti-EpCAM, anti-Salmonella, anti-E. coli, and anti-Listeria antibodies from different suppliers. Their properties were compared for their ability to interact specifically with antigen and/or non-target structures and the best-suited antibody for the intended application was identified.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/EF17_048%2F0007421" target="_blank" >EF17_048/0007421: Strengthening interdisciplinary cooperation in research of nanomaterials and their effects on living organisms (NANOBIO)</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical Methods: advancing methods and applications

ISSN

1759-9660

e-ISSN

1759-9679

Volume of the periodical

13

Issue of the periodical within the volume

35

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

3874-3884

UT code for WoS article

000687267500001

EID of the result in the Scopus database

2-s2.0-85115395353