Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39896843" target="_blank" >RIV/00216275:25310/14:39896843 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/14:00427443
Result on the web
<a href="http://dx.doi.org/10.1002/elps.201300196" target="_blank" >http://dx.doi.org/10.1002/elps.201300196</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201300196" target="_blank" >10.1002/elps.201300196</a>
Alternative languages
Result language
angličtina
Original language name
Application of an improved magnetic immunosorbent in an Ephesia chip designed for circulating tumor cell capture
Original language description
In this study, we describe a particular step in developing a microfluidic device for capture and detection of circulating tumor cells?specifically the preparation of an immunosorbent for implementation into the separation chip. We highlight some of the most important specifics connected with superparamegnetic microspheres for microfluidic purposes. Factors such as nonspecific adsorption on microfluidic channels, interactions with model cell lines, and tendency to aggregation were investigated. Poly(glycidyl methacrylate) microspheres with carboxyl groups were employed for this purpose. To address the aforementioned challenges, the microspheres were coated with hydrazide-PEG-hydrazide, and subsequently anti-epithelial cell adhesion molecule (EpCAM) antibody was immobilized. The prepared anti-EpCAM immunosorbent was pretested using model cell lines with differing EpCAM density (MCF7, SKBR3, A549, and Raji) in a batchwise arrangement. Finally, the entire system was implemented and studied
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/7E09109" target="_blank" >7E09109: Integrated Micro-Nano-Opto Fluidic systems for high-content diagnosis and studies of rare cancer cells</a><br>
Continuities
R - Projekt Ramcoveho programu EK
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
—
Volume of the periodical
35
Issue of the periodical within the volume
2-3
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
323-329
UT code for WoS article
000331899400012
EID of the result in the Scopus database
—