Use of magnetic hydrazide-modified polymer microspheres for enrichment of Francisella tularensis glycoproteins
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F12%3A00373792" target="_blank" >RIV/61389013:_____/12:00373792 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G44__/12:43874661
Result on the web
<a href="http://dx.doi.org/10.1039/c2sm07036g" target="_blank" >http://dx.doi.org/10.1039/c2sm07036g</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/c2sm07036g" target="_blank" >10.1039/c2sm07036g</a>
Alternative languages
Result language
angličtina
Original language name
Use of magnetic hydrazide-modified polymer microspheres for enrichment of Francisella tularensis glycoproteins
Original language description
The use of magnetic microspheres containing functional groups is nowadays among state-of-art separation methodologies owing to an ease of manipulation, a speed of separation, and a minimum of non-specific protein adsorption. In the present study, novel magnetic hydrazide-modified poly(2-hydroxyethyl methacrylate) (PHEMA) microspheres were developed using a multi-step swelling and polymerization method with subsequent precipitation of magnetic iron oxides within the pores of the particles. The microspheres had a regular shape, size of 4 ?m and contained 0.18 mmol hydrazide groups per g; the magnetic microspheres were employed for specific enrichment of Francisella tularensis glycoproteins. Effectiveness of the newly prepared magnetic microspheres for glycoprotein enrichment was proved by comparison with commercial hydrazide-functionalized microparticles.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA203%2F09%2F0857" target="_blank" >GA203/09/0857: New analytical approaches for identification of proteins with significant attributes for virulence and pathogenity of bacteria</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Soft Matter
ISSN
1744-683X
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
9
Country of publishing house
GB - UNITED KINGDOM
Number of pages
12
Pages from-to
2775-2786
UT code for WoS article
000300048300025
EID of the result in the Scopus database
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