Evaluation of colorimetric BCA-based quantification of hydrazide groups on magnetic particles

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F18%3A00489057" target="_blank" >RIV/61389013:_____/18:00489057 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1155/2018/5492893" target="_blank" >http://dx.doi.org/10.1155/2018/5492893</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2018/5492893" target="_blank" >10.1155/2018/5492893</a>

Result language

angličtina

Original language name

Evaluation of colorimetric BCA-based quantification of hydrazide groups on magnetic particles

Original language description

Magnetic micro- and nanoparticles (MPs) are considered to provide excellent solid support for many immunoanalytical and bioaffinity applications, particularly when they contain hydrazide groups available for site-specific immobilization of various glycoproteins, such as immunoglobulin G and enzymes. To prepare a highly active bioaffinity carrier with sufficient binding capacity, knowledge as to the type and concentration of functional groups used for ligand binding is crucial. Described here is a simple, nontoxic method for rapid estimation of hydrazide functional groups bound to MPs using bicinchoninic acid (BCA). BCA kits are routinely used for colorimetric detection and quantification of total protein in liquid samples. In this study, the BCA reagent was applied for quantification of hydrazide groups on MPs. The approach was carried out using an adipic acid dihydrazide (ADH) solution and subsequently using various hydrazide-containing magnetic and nonmagnetic carriers differing in the density of hydrazide groups. The BCA test’s results obtained on the MPs were compared with those from conventional amino and hydrazide group quantification by the 2,4,6-trinitrobenzenesulfonic acid (TNBS) test.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10404 - Polymer science

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Spectroscopy

ISSN

2314-4920

e-ISSN

—

Volume of the periodical

2018

Issue of the periodical within the volume

19 April

Country of publishing house

US - UNITED STATES

Number of pages

5

Pages from-to

1-5

UT code for WoS article

000431242100001

EID of the result in the Scopus database

2-s2.0-85059173041