Effects of fatty acyl chain lengths, unsaturation degree, concentration and used matrix on phosphatidylcholine responses in MALDI-MS

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F14%3A39899345" target="_blank" >RIV/00216275:25310/14:39899345 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Effects of fatty acyl chain lengths, unsaturation degree, concentration and used matrix on phosphatidylcholine responses in MALDI-MS
Original language description
Nevertheless, it is important to investigate these effects in more details for better understanding of lipids properties and for accurate quantitation. Methods Sets of PCs with fixed fatty acyl (14:0, 17:0 or 18:1) in sn-1 position and varied in length and saturation degree of fatty acyls in sn-2 position of glycerol backbone were synthesized. Samples with various concentrations of lipids were mixed with several MALDI matrices including DHB, 9-aminoacridine and diaminonaphthalene. Results MALDI-MS investigation of synthesized PC sets revealed the dependence of the intensity of lipid-related peaks on fatty acyl lengths. The character of the dependence was changed depending on used matrices and concentrations of lipids. In case of DHB matrix, the dependence was not monotonic. Peak intensities increased with the chain length increasing for samples with higher lipids concentrations and decreased for lower concentrations. The fact of lipid peaks decrease for longer fatty acyl chains for sam
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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