Magnetic beads-based electrochemical immunosensor for monitoring Magnetic beads-based electrochemical immunosensor for monitoring allergenic food proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39899542" target="_blank" >RIV/00216275:25310/15:39899542 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.ab.2015.04.037" target="_blank" >http://dx.doi.org/10.1016/j.ab.2015.04.037</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ab.2015.04.037" target="_blank" >10.1016/j.ab.2015.04.037</a>

Result language

angličtina

Original language name

Magnetic beads-based electrochemical immunosensor for monitoring Magnetic beads-based electrochemical immunosensor for monitoring allergenic food proteins

Original language description

Screen-printed platinum electrodes as transducer and magnetic beads as solid phase were combined to develop a particle-based electrochemical immunosensor for monitoring the serious food allergen ovalbumin. The standard arrangement of enzyme-linked immunosorbent assay became the basis for designing the immunosensor. A sandwich-type immunocomplex was formed between magnetic particles functionalized with specific anti-ovalbumin immunoglobulin G and captured ovalbumin molecules, and secondary anti-ovalbuminantibodies conjugated with the enzyme horseradish peroxidase were subsequently added as label tag. The electrochemical signal proportional to the enzymatic reaction of horseradish peroxidase during the reduction of hydrogen peroxide with thionine as electron mediator was measured by linear sweep voltammetry. The newly established method of ovalbumin detection exhibits high sensitivity suitable for quantification in the range of 11 to 222 nM and a detection limit of 5 nM. Magnetic beads-

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GAP206%2F12%2F0381" target="_blank" >GAP206/12/0381: Development of ultrasensitive electrochemical immunosensors for early detection of clinically significant biomarkers of malignant diseases</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical Biochemistry

ISSN

0003-2697

e-ISSN

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Volume of the periodical

484

Issue of the periodical within the volume

September

Country of publishing house

US - UNITED STATES

Number of pages

5

Pages from-to

4-8

UT code for WoS article

000357967500002

EID of the result in the Scopus database

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