Magnetic beads-based electrochemical immunosensor for monitoring allergenic food proteins

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F15%3A00445033" target="_blank" >RIV/61389013:_____/15:00445033 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.ab.2015.04.037" target="_blank" >http://dx.doi.org/10.1016/j.ab.2015.04.037</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ab.2015.04.037" target="_blank" >10.1016/j.ab.2015.04.037</a>

Result language
angličtina
Original language name
Magnetic beads-based electrochemical immunosensor for monitoring allergenic food proteins
Original language description
Screen-printed platinum electrodes as transducer and magnetic beads as solid phase were combined to develop a particle-based electrochemical immunosensor for monitoring the serious food allergen ovalbumin. The standard arrangement of enzyme-linked immunosorbent assay became the basis for designing the immunosensor. A sandwich-type immunocomplex was formed between magnetic particles functionalized with specific anti-ovalbumin immunoglobulin G and captured ovalbumin molecules, and secondary anti-ovalbuminantibodies conjugated with the enzyme horseradish peroxidase were subsequently added as label tag. The electrochemical signal proportional to the enzymatic reaction of horseradish peroxidase during the reduction of hydrogen peroxide with thionine as electron mediator was measured by linear sweep voltammetry. The newly established method of ovalbumin detection exhibits high sensitivity suitable for quantification in the range of 11 to 222 nM and a detection limit of 5 nM. Magnetic beads-
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
<a href="/en/project/GAP206%2F12%2F0381" target="_blank" >GAP206/12/0381: Development of ultrasensitive electrochemical immunosensors for early detection of clinically significant biomarkers of malignant diseases</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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