Alkaline phosphatase labeled antibody-based electrochemical biosensor for sensitive HE4 tumor marker detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39899831" target="_blank" >RIV/00216275:25310/15:39899831 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.elecom.2015.06.014" target="_blank" >http://dx.doi.org/10.1016/j.elecom.2015.06.014</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.elecom.2015.06.014" target="_blank" >10.1016/j.elecom.2015.06.014</a>
Alternative languages
Result language
angličtina
Original language name
Alkaline phosphatase labeled antibody-based electrochemical biosensor for sensitive HE4 tumor marker detection
Original language description
Quantitative determination of the serum level of human epididymis protein 4 (HE4), a tumor marker for ovarian carcinoma, has come to the fore of interest mainly due to the possibility for its detection in early stages of the disease when the sensing of other biomarkers is limited. We present a simple ELISA based approach for rapid HE4 detection including its immunomagnetic capturing accompanied by sensitive electrochemical detection of the electroactive product formed after enzymatic conversion of the substrate by alkaline phosphatase used as a label of anti-HE4 IgG. The proposed immunosensor offers stability in time and due to excellent limit of detection at 6.8 fM HE4, and limit of quantification of 23 fM meets the requirements for the early detection of this biomarker.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CG - Electrochemistry
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/GAP206%2F12%2F0381" target="_blank" >GAP206/12/0381: Development of ultrasensitive electrochemical immunosensors for early detection of clinically significant biomarkers of malignant diseases</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrochemistry Communications
ISSN
1388-2481
e-ISSN
—
Volume of the periodical
59
Issue of the periodical within the volume
October
Country of publishing house
US - UNITED STATES
Number of pages
4
Pages from-to
1-4
UT code for WoS article
000361251100001
EID of the result in the Scopus database
—