Sensitive biosensor for detection oncogenic miRNA-21

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU134936" target="_blank" >RIV/00216305:26620/19:PU134936 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/19:43917158
Result on the web
<a href="https://mendelnet.cz/artkey/mnt-201901-0130_Sensitive-biosensor-for-detection-oncogenic-miRNA-21.php?back=/magno/mnt/2019/mn1.php?secid=4" target="_blank" >https://mendelnet.cz/artkey/mnt-201901-0130_Sensitive-biosensor-for-detection-oncogenic-miRNA-21.php?back=/magno/mnt/2019/mn1.php?secid=4</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Sensitive biosensor for detection oncogenic miRNA-21
Original language description
The aim of this study was to prepare a sensitive electrochemical biosensor for detection of oncogenic miRNA-21 as a potential biomarker for early detection of cancer. MicroRNAs (miRNAs) are small noncoding RNA, which play an important role in the regulation of gene expression. miRNAs are present in extracellular fluids like urine, serum, etc. in extremely low concentration. It is important for the biosensor to detect extremely low concentrations of specific miRNA as potential biomarkers. The aim of this experiment was designing, preparing and optimizing a sensitive and selective biosensor for the detection of miRNA-21. A biosensor was prepared to detect the lowest concentration of miRNA-21 in the sample. The linear concentration range for the calibration curve was 1 fM to 10 nM. LOD was 1 fM and obtained from the regression equation was 3.2 zM and LOQ was 10.8 zM. Subsequently, prepared biosensor was measured in artificial urine (AU) samples to verify the functioning of the sensor in induced real conditions. The results showed a minimal effect of the matrix for the determination of the target miRNA.
Czech name
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Czech description
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Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2019 Proceedings of 26th International PhD Students Conference
ISBN
978-80-7509-688-3
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
676-681
Publisher name
Mendel University in Brno
Place of publication
Neuveden
Event location
Brno
Event date
Nov 6, 2019
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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