Highly selective and sensitive electrochemical biosensor based on electrochemically reduced graphene oxide for detection of miRNA as a cancer biomarker

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43917970" target="_blank" >RIV/62156489:43210/20:43917970 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/20:PU136652

Result on the web

<a href="https://doi.org/10.37904/nanocon.2019.8615" target="_blank" >https://doi.org/10.37904/nanocon.2019.8615</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2019.8615" target="_blank" >10.37904/nanocon.2019.8615</a>

Result language

angličtina

Original language name

Highly selective and sensitive electrochemical biosensor based on electrochemically reduced graphene oxide for detection of miRNA as a cancer biomarker

Original language description

Nowadays, cancer is one of the most outrageous diseases affecting millions of people throughout the world. In this study, the sensitive biosensor for miRNA-21 detection was developed. Through a facile sulfonamide coupling reaction a miRNA-21 specific DNA hybridization probe was immobilized onto a glassy carbon electrode (GCE) modified with electrochemical reduced graphene oxide (erGO). miRNA is a small non-coding RNA molecule which plays an important role in gene expression. The level of miRNA in extracellular fluids varying in the presence of cancer and it can serve as creates a potential biomarker for early detection of cancer. A higher levelcancer. An elevated level of miRNA-21 represents a common feature of pathological cell growth or cell stress and is connected with almost all types of cancer and with cardiovascular diseases, as well.The aim of this study was improve the electrochemical properties by using erGO as a modification for better sensitivity of the developed biosensor. After the modification with erGO, 1-amino-2-naphthol-4-sulfonic acid (AN-SO3-) was electrodeposited on the GCE surface.Then the GCE was subsequently modified by electrochemical deposition 1-amino-2-naphthol-4-sulfonic acid (AN-SO3-) on the GCE surface. Finally, the amino terminated capture probe was immobilized on the GCE via the sulfonamide coupling reaction. The developed biosensor for the detection of specific miRNA sequence was optimized and modified for using in further experiments.

Czech name

—

Czech description

—

Type

D - Article in proceedings

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

NANOCON 2019: Conference Proceedings

ISBN

978-80-87294-95-6

ISSN

2694-930X

e-ISSN

—

Number of pages

6

Pages from-to

385-390

Publisher name

Tanger Ltd.

Place of publication

Ostrava

Event location

Brno

Event date

Oct 16, 2019

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000664115400064