Development of electrochemical biosensor for direct histamine detection by using selective peptides as biorecognition element

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43919619" target="_blank" >RIV/62156489:43210/20:43919619 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2020/mnet_2020_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Development of electrochemical biosensor for direct histamine detection by using selective peptides as biorecognition element
Original language description
The level of histamine (HIS) in different food sources is critical as an indicator of food quality and safety. In this context, the proper monitoring of HIS in food is necessary. Electrochemical biosensors, with the possibility of fast, sensitive, and portable technology, represent a new promising approach for direct detection of HIS. Moreover, peptides as biorecognition elements are promising due to their unique properties such as good stability, specific and selective binding to various biomolecules. In this study, the fabrication and optimization of an electrochemical biosensor for direct detection of HIS based-on peptides as recognition elements, is presented. The specific HIS binding peptides (HBP) were immobilized via a sulphonamide bond on 1-amino-2-naphthol-4-sulfonic acid (ANSA), which was electrodeposited on a glassy carbon electrode (GCE). Furthermore, the optimization of the surface coverage with the peptide probe as well as the effect of the pH conditions for HIS binding was investigated. The measured concentrations were from 10 nM (10MINUS SIGN 8 M) to 1 mM (10MINUS SIGN 3 M) and LOD was calculated as 6.53 nM and LOQ as 21.75 mM.
Czech name
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Czech description
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Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2020: Proceedings of International PhD Students Conference
ISBN
978-80-7509-765-1
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
578-583
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 11, 2020
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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