Thionine-Modified Poly(glycidyl methacrylate) Nanospheres as Labels of Antibodies for Biosensing Applications
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39899836" target="_blank" >RIV/00216275:25310/15:39899836 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/15:00450848
Result on the web
<a href="http://pubs.acs.org/doi/abs/10.1021/acsami.5b08469" target="_blank" >http://pubs.acs.org/doi/abs/10.1021/acsami.5b08469</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acsami.5b08469" target="_blank" >10.1021/acsami.5b08469</a>
Alternative languages
Result language
angličtina
Original language name
Thionine-Modified Poly(glycidyl methacrylate) Nanospheres as Labels of Antibodies for Biosensing Applications
Original language description
Monodisperse poly(glycidyl methacrylate) (PGMA) nanospheres were obtained by emulsifier-free emulsion polymerization and characterized by physicochemical methods. The effects of various reaction parameters on the particle properties were investigated. The particle size was controlled in the range of 350MINUS SIGN 420 nm. To introduce carboxyl groups, the PGMA nanospheres were hydrolyzed and oxidized with KMnO4. Subsequently, the enzyme horseradish peroxidase (HRP) and the electron mediator thionine were covalently attached to the PGMA nanospheres to obtain an antibody indicator suitable for enzyme-based electrochemical immunosensors. Combined HRP and thionine binding to the nanospheres had beneficial effects for the labeling efficiency and at the same time prevented the formation of soluble electron mediators.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Applied Materials & Interfaces
ISSN
1944-8244
e-ISSN
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Volume of the periodical
7
Issue of the periodical within the volume
44
Country of publishing house
US - UNITED STATES
Number of pages
6
Pages from-to
"24926?24931"
UT code for WoS article
000364726400062
EID of the result in the Scopus database
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