Determination of seminal plasma malondialdehyde by high-performance liquid chromatography in smokers and non-smokers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900025" target="_blank" >RIV/00216275:25310/15:39900025 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.4149/BLL_2015_004" target="_blank" >http://dx.doi.org/10.4149/BLL_2015_004</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4149/BLL_2015_004" target="_blank" >10.4149/BLL_2015_004</a>
Alternative languages
Result language
angličtina
Original language name
Determination of seminal plasma malondialdehyde by high-performance liquid chromatography in smokers and non-smokers
Original language description
Objectives: The aim of this study was to establish and validate a high-performance liquid chromatography method for the determination of malondialdehyde in seminal plasma in smokers and non-smokers and to find possible differences between the two groups.Background: Malondialdehyde is used as a diagnostic marker of lipid peroxidation and indicator of oxidative stress. Smoking is suspected to be responsible for an increase in its level. Malondialdehyde has been thought to have cytotoxic and damaging effects. Methods: Semen samples were obtained from male partners of couples requesting a fertility evaluation. Malondialdehyde was derivatized with 2-thiobarbituric acid. The malondialdehyde-2-thiobarbituric acid complex was determined by liquid chromatography with fluorescence detection. The mobile phase consisted of 20% ethanol in 25-mmol/L potassium dihydrogenphosphate (v/v), pH 6.00 +- 0.05. Results: Analytical performance was satisfactory. Malondialdehyde levels were as follows: 1.50 +-
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bratislava Medical Journal - Bratislavske Lekarske Listy
ISSN
0006-9248
e-ISSN
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Volume of the periodical
116
Issue of the periodical within the volume
1
Country of publishing house
SK - SLOVAKIA
Number of pages
5
Pages from-to
20-24
UT code for WoS article
000349275900004
EID of the result in the Scopus database
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