Determination of Total Glutathione in Dried Blood Spot Samples Using a High-Performance Liquid Chromatography
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900027" target="_blank" >RIV/00216275:25310/15:39900027 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1093/chromsci/bmu135" target="_blank" >http://dx.doi.org/10.1093/chromsci/bmu135</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/chromsci/bmu135" target="_blank" >10.1093/chromsci/bmu135</a>
Alternative languages
Result language
angličtina
Original language name
Determination of Total Glutathione in Dried Blood Spot Samples Using a High-Performance Liquid Chromatography
Original language description
A method is described for the determination of total glutathione (TGSH) in dried blood spot (DBS) samples using high-performance liquid chromatography with fluorescence detection. Whole blood and DBS samples were obtained from a group of blood donors. After GSH reduction with dithiothreitol and protein precipitation with ethanol, the samples were derivatized with naphthalene-2,3-dicarboxaldehyde to form a very stable, highly fluorescent derivative. For the separation, a reversed phase HPLC method was used. The mixture of ethanol and deionized water (8 : 92, v/v) was used as a mobile phase. The analytical performance of this method was satisfactory: the intra-and interassay coefficients of variation were below 10%. Quantitative recoveries from spiked DBS samples were between 98.3 and 103.6%. The presented method is inexpensive and suitable for clinical testing purposes.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of chromatographic science
ISSN
0021-9665
e-ISSN
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Volume of the periodical
53
Issue of the periodical within the volume
6
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
879-885
UT code for WoS article
000361837600007
EID of the result in the Scopus database
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