Electrochemical Verification of Enzyme/QDs Labelled Antibodies Functionality: An Essential Step in ELISA Methods

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900382" target="_blank" >RIV/00216275:25310/15:39900382 - isvavai.cz</a>
Result on the web
<a href="http://www.bestservis.eu/upload/file/Sbornik_metody15.pdf" target="_blank" >http://www.bestservis.eu/upload/file/Sbornik_metody15.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Electrochemical Verification of Enzyme/QDs Labelled Antibodies Functionality: An Essential Step in ELISA Methods
Original language description
In development of immunoassays for biomarkers of such diseases, the availability of secondary antibodies conjugated with suitable label which could be utilized for highly sensitive electrochemical detection is limited. We established efficient in-house labelling procedure for conjugation of antibodies with CdSe/ZnS quantum dots (QDs) based on antigen modified magnetic microparticles serving as a solid anchor. QLISA based immunosensors achieve higher sensitivity comparing to ELISA based biosensors. Alsoin case of labelling of antibodies with alkaline phosphatase (ALP) we present a simple verification of labelling protocol efficiency based on electrochemical detection.
Czech name
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Czech description
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Project
<a href="/en/project/GA15-16549S" target="_blank" >GA15-16549S: Development of ultrasensitive magneto-immunoassay based on quantum dots/antibody probes for simultaneous electrochemical detection of cancer markers.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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