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Verification of antibody labelling efficiency as an important step in ELISA/QLISA development

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F16%3A39900017" target="_blank" >RIV/00216275:25310/16:39900017 - isvavai.cz</a>

  • Result on the web

    <a href="http://link.springer.com/article/10.1007/s00706-015-1598-1" target="_blank" >http://link.springer.com/article/10.1007/s00706-015-1598-1</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s00706-015-1598-1" target="_blank" >10.1007/s00706-015-1598-1</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Verification of antibody labelling efficiency as an important step in ELISA/QLISA development

  • Original language description

    A simple and effective method for validation of antibody labelling procedure as well as for optimal conjugate dilution assessment based on electrochemical detection is presented. Enzyme alkaline phosphatase and core-shell CdSe/ZnS quantum dots are used as sensitive tag exploitable as detection tool of antibody-antigen complex in magnetic microbead-based immunoassays. Square-wave voltammetry for detection of the electroactive product that is induced by conversion of appropriate substrate by alkaline phosphatase and square-wave anodic-stripping voltammetry for signal of Cd in case of quantum dots, respectively, was elected. Anti-Apo E IgG antibodies were selected as model molecules for method optimizations.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA15-16549S" target="_blank" >GA15-16549S: Development of ultrasensitive magneto-immunoassay based on quantum dots/antibody probes for simultaneous electrochemical detection of cancer markers.</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Monatshefte fur Chemie

  • ISSN

    0026-9247

  • e-ISSN

  • Volume of the periodical

    147

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    AT - AUSTRIA

  • Number of pages

    5

  • Pages from-to

    69-73

  • UT code for WoS article

    000367521400010

  • EID of the result in the Scopus database