Verification of antibody labelling efficiency as an important step in ELISA/QLISA development
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F16%3A39900017" target="_blank" >RIV/00216275:25310/16:39900017 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/article/10.1007/s00706-015-1598-1" target="_blank" >http://link.springer.com/article/10.1007/s00706-015-1598-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00706-015-1598-1" target="_blank" >10.1007/s00706-015-1598-1</a>
Alternative languages
Result language
angličtina
Original language name
Verification of antibody labelling efficiency as an important step in ELISA/QLISA development
Original language description
A simple and effective method for validation of antibody labelling procedure as well as for optimal conjugate dilution assessment based on electrochemical detection is presented. Enzyme alkaline phosphatase and core-shell CdSe/ZnS quantum dots are used as sensitive tag exploitable as detection tool of antibody-antigen complex in magnetic microbead-based immunoassays. Square-wave voltammetry for detection of the electroactive product that is induced by conversion of appropriate substrate by alkaline phosphatase and square-wave anodic-stripping voltammetry for signal of Cd in case of quantum dots, respectively, was elected. Anti-Apo E IgG antibodies were selected as model molecules for method optimizations.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA15-16549S" target="_blank" >GA15-16549S: Development of ultrasensitive magneto-immunoassay based on quantum dots/antibody probes for simultaneous electrochemical detection of cancer markers.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Monatshefte fur Chemie
ISSN
0026-9247
e-ISSN
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Volume of the periodical
147
Issue of the periodical within the volume
1
Country of publishing house
AT - AUSTRIA
Number of pages
5
Pages from-to
69-73
UT code for WoS article
000367521400010
EID of the result in the Scopus database
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