An integrated microfluidic chip for immunocapture, preconcentration and separation of ?-amyloid peptides

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F15%3A39900560" target="_blank" >RIV/00216275:25310/15:39900560 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1063/1.4931394" target="_blank" >http://dx.doi.org/10.1063/1.4931394</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1063/1.4931394" target="_blank" >10.1063/1.4931394</a>

Result language

angličtina

Original language name

An integrated microfluidic chip for immunocapture, preconcentration and separation of ?-amyloid peptides

Original language description

We present an integrated microfluidic chip for detection of ?-amyloid (A?) peptides. A? peptides are major biomarkers for the diagnosis of Alzheimer's disease (AD) in its early stages. This microfluidic device consists of three main parts: (1) An immunocapture microcolumn based on self-assembled magnetic beads coated with antibodies specific to A? peptides, (2) a nano-porous membrane made of photopolymerized hydrogel for preconcentration, and (3) a microchip electrophoresis (MCE) channel with fluorescent detection. Sub-milliliter sample volume is either mixed off-chip with antibody coated magnetic beads and injected into the device or is injected into an already self-assembled column of magnetic beads in the microchannel. The captured peptides on the beads are then electrokinetically eluted and re-concentrated onto the nano-membrane in a few nano-liters. By integrating the nano-membrane, total assay time was reduced and also off-chip re-concentration or buffer exchange steps were not n

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/7E12083" target="_blank" >7E12083: NAnosystems for the early DIagnosis of NEurodegenerative Diseases</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomicrofluidics

ISSN

1932-1058

e-ISSN

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Volume of the periodical

9

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

054117

UT code for WoS article

000364407300029

EID of the result in the Scopus database

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