Label-free bead-based metallothionein electrochemical immunosensor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F15%3A00087442" target="_blank" >RIV/00216224:14110/15:00087442 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/15:PU118478 RIV/62156489:43210/15:43907686

Result on the web

<a href="http://dx.doi.org/10.1002/elps.201500069" target="_blank" >http://dx.doi.org/10.1002/elps.201500069</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201500069" target="_blank" >10.1002/elps.201500069</a>

Result language

angličtina

Original language name

Label-free bead-based metallothionein electrochemical immunosensor

Original language description

A novel microfluidic label-free bead-based metallothionein immunosensors was designed. To the surface of superparamagnetic agarose beads coated with protein A, polyclonal chicken IgY specifically recognizing metallothionein (MT) were immobilized via rabbit IgG. The Brdicka reaction was used for metallothionein detection in a microfluidic printed 3D chip. Assembled chip consisted of a single copper wire coated with a thin layer of amalgam as working electrode. Optimization of MT detection using designed microfluidic chip was performed in stationary system as well as in the flow arrangement at various flow rates (0-1800 mikroL/min). In stationary arrangement it is possible to detect MT concentrations up to 30 ng/mL level, flow arrangement allows reliable detection of even lower concentration (12.5 ng/mL). The assembled miniature flow chip was subsequently tested for the detection of MT elevated levels (at approx. level 100 mikrog/mL) in real samples of patients with cancer.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

36

Issue of the periodical within the volume

16

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

1894-1904

UT code for WoS article

000360055300014

EID of the result in the Scopus database

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