Optimisation of Analytical Method for Extraction and Chromatographic Analysis of Phenolic Compounds in Brewing Malts

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F16%3A39901552" target="_blank" >RIV/00216275:25310/16:39901552 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Optimisation of Analytical Method for Extraction and Chromatographic Analysis of Phenolic Compounds in Brewing Malts

Original language description

Phenolic compounds are one of the most biologically active substances with positive impact on the human health. These are substances of the plant origin protecting plants against adverse environmental effects. In their structure an aromatic ring containing one or more hydroxyl groups are occurring. These compounds are divided by the structure into groups known as flavonoids, phenolic acids, stilbenes and lignans. Beer is well known as a rich source of phenolic compounds from which 80% comes from malt. First part of this study is focused on the optimisation of chromatographic process to separate eleven phenolic substances using gradient elution. Optimized conditions of separation were furthermore used for analysis of phenolic substances in congress wort extracts, prepared from samples of barley and wheat malts. Samples of congress wort were brewed by well-defined procedure commonly used as standard methodology in brewing. Through the time of saccharification an amylolytic enzymes activity in malt is determined. Saccharification is tested 10 minutes after reaching the temperature point of mash at 70 oC through the iodine test. For pre-concentration of analytes from congress wort, the microextraction by packed sorbent method (MEPS) was chosen. The most used sorbents for MEPS are silica-based (C18, C8, C2, Silica, or mixed sorbent C8 + SCX). First experiments have confirmed that for tested compounds extraction, the C18 sorbent is the best choice. Choosing the MEPS method allowed not only minimisation of sample and solvents consumptions, but also pre-concentration and sampling in one-step. After separation by HPLC using gradient elution, target phenolic compounds were detected by diode array detector (DAD). Its advantage is that it could select the suitable wavelength for analysis of individual components. Second type of detector used in this study was electrochemical coulometric detector (ECD), which is selective for substances containing hydroxyl groups.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

23th Young Investigators' Seminar on Analytical Chemistry : book of proceedings

ISBN

978-86-7031-415-3

ISSN

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e-ISSN

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Number of pages

5

Pages from-to

1-5

Publisher name

University of Novi Sad

Place of publication

Novi Sad

Event location

Novi Sad

Event date

Jun 28, 2016

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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